Zfisher is a web application to check the coding potentials of dubious genes in sequenced genomes based on the Z curve method. In the Zfisher, 33 features corresponding to single nucleotide and dinulceotide frequencies are used to describe the sequences. The Fisher linear discrimination algorithm is used to optimally differentiate protein-coding and non-coding sequences.
To run the tool, two types of sets are needed. First, DNA sequences of function-known genes would be uploaded or pasted into the upper window and they are used as training set of the method. Second, DNA sequences of doubious genes could be submitted to the lower window. Finally, the result will be shown in one new webpage several minutes after you clicking the Run button. In the result, five-fold validation and self-test based on the whole training set will be given, and details of the predicted non-coding ORFs are also provided.